Expression of hepatocyte growth factor in the inflammatory periodontal tissue of rats during orthodontic tooth movement

doi:10.3969/j.issn.2095-4344.2014.15.014

Abstract

Abstract:

BACKGROUND: The cytokine has an effect of immunoregulation and immediate induction in the reconstruction of periodontal tissue. At present, the role and mechanism of hepatocyte growth factor involving the reconstruction of periodontal tissue under orthodontic force are still unclear.
OBJECTIVE: To explore the mechanism underlying hepatocyte growth factor in the tooth movement and periodontal tissues remodeling under the inflammation periodontal tissue condition.
METHODS: Thirty Sprague-Dawley rats, aged 8 weeks, were used to establish periodontitis model. The obtained model was randomly divided into two groups: inflammatory control group and inflammatory force group. In the force group, rats were treated with the fixed orthodontic appliance by 50 g forces in the maxillary first molars. After 1, 3, 5, 7 and 14 days of tooth movement, five rats were sacrificed respectively. Hematoxylin-eosin staining and immunohistochemistry methods were used to analyze the expression and distribution of hepatocyte growth factor in the periodontium for rats at different tooth movement stages.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining results showed that, remodeling of periodontal tissues existed in all groups. The immunohistochemical results showed that hepatocyte growth factor had positive expression in periodontal tissue, and the distribution was even in the control group. In the force group, hepatocyte growth factor expression was increased and reached the peak on day 5, then began to decline. Osteoblast, fibroblast and osteoclast were strongly expressed. The findings indicate that, hepatocyte growth factor is involved in the periodontal tissues remodeling under orthodontic force, and inflammation can increase the expression of hepatocyte growth factor in periodontal tissue.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: periodontitis, hepatocyte growth factor, models, animal

CLC Number:

R318

Liu Hong, Mi Cong-bo, Zhu Jun. Expression of hepatocyte growth factor in the inflammatory periodontal tissue of rats during orthodontic tooth movement[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(15): 2377-2382.

Figures/Tables 2

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